cDNA and PCR - enzimes (Mar/27/2007 )
which one do you think is better?
I need to do cDNA of RNA so that I can then do a PCR to amplify two transcripts of 1.9 and 2.7Kb.
the RT is using an oligo dT, which enzyme should I used? ImPromII or M-MLV? and for the PCR?
The problem is that maybe the sequence is quite long for standard enzimes.
what do you recommend?
we are using RevertAid M-MuLV reverse Transcriptase.(EP0441)and following fermentas protocol.