bone marrow isolation - (Mar/13/2007 )

Hi,

How can I isolate leucocytes from bone marrow.
Thanks

-BBSRILS-

hi,

so simple!
cut the 2 edges of the bone, flush the inside with medium (with a needle and seringue), lyse erythrocytes, and wash the cells in medium

Sebastien

-tryptofan-

QUOTE (tryptofan @ Mar 14 2007, 02:59 AM)

hi,

so simple!
cut the 2 edges of the bone, flush the inside with medium (with a needle and seringue), lyse erythrocytes, and wash the cells in medium

Sebastien

Thanks. Supposedly I want to seperate the lymphocytes and the granulocytes from bone marrow samples, can I follow the ficoll procedure or some other precautions needs to be taken.

-BBSRILS-

sorry, I have not read your question correctly
I have no experience with Ficoll separation on BM extracts, but I suppose that there is no reason for special precautions...
Seb

-tryptofan-

As far as I'm aware, ficoll is flexible regarding the source of the cells. The most important parameter is the density of the cells.

-swanny-