confused by a simple question on concentration caculation - (Mar/12/2007 )
I am now very perplexed by a simple question of concentration caculation.
I need to make 14% solution of reagent A in PBS buffer. The reagent A are poweders.
So, should I weight 14g reagent A and dissolve it in 100 ml PBS?
Thanks a lot!~
dissolve it with pbs to a final volume of 100 ml.
Dissolve in half the volume, then fill up to 100 mL.
That'll give you 14%w/v concentration, no idea if you need w/v or w/w - they are slightly different.
14% w/w means 14 g of A in 100 g of everything (A+PBS)...