3,3'-Diaminobenzidine tetrahydrochloride - (Mar/06/2007 )

Hey there

In the lab that I had the other day I added the above to my slide..3,3'-Diaminobenzidine tetrahydrochloride--why is this the case--from what I gather from the instructions we were given it's a substrate that binds to the peroxidase or something like that which gives a brown colour and it is that brown colour (stain) we are able to view with the light microscope? right? (probably not)


Cheers
biology_0er

your questions are making me feel old (its fifteen years since ive done this)
as i remember it the 3,3... is shortened to dab and you are right it allows you to visualise the peroxidase by turning it brown

right !!!

so what is correct..

1)-peroxidase is the substate and DAB is the enzyme

or

2)DAB is the substrate and peroxidase is the enzyme

I think its (1) because we added the peroxidase before the DAB

Cheers
biology_06er

that is one of the strangest logic statement I have read.

The order at which one adds one compound to another does not determine its nature. Something doesn't become a catalyst if I add it last.

3,3'-Diaminobenzidine is basically two benzene rings linked together with four amine groups sticking out. Yes there are transitional metal salts with simple structures that work as catalyst, but it is kind of a strech of the imagination to call 3,3'-Diaminobenzidine an enzyme.

The enzyme is peroxidase (the name kind of hints that it is an enzyme)

that is one of the strangest logic statement I have read.

The order at which one adds one compound to another does not determine its nature. Something doesn't become a catalyst if I add it last.

3,3'-Diaminobenzidine is basically two benzene rings linked together with four amine groups sticking out. Yes there are transitional metal salts with simple structures that work as catalyst, but it is kind of a strech of the imagination to call 3,3'-Diaminobenzidine an enzyme.

The enzyme is peroxidase (the name kind of hints that it is an enzyme)