Southern set-up - help and tips (Jun/24/2003 )
I' ve a problem, I have to set-up a Southern blot to study a new gene and I don't know how to choose the informative restriction enzyme.
Can some body give me some tips to design my southern blot?
do u know the all dna sequence? if u do so choose enzyme that cut at your gene limits (if your gene is more then 15 kp so cut just part of him). (know what is the size of this fragment) use probe that is more then 500 bp from your gene do your probe by pcr or cut and insert it to a vector. the rest of the protocol u can find on this site.
good luck yossi