Molecular weight and Centrifugation: Help! - (Mar/05/2007 )
Question;
Is there a chart/table that lists the molecular weight of particles that sediment at different/certain RCF speeds?
ie. what G force sediments a 340 kDa protein?
or, at 100 000 G what molecules are we expecting to sediment?
Furthermore, at a high G like 100 000, what is the lightest molecular weight that will sediment?
Any help/ suggestions is greatly appreciated
-Hales-
QUOTE (Hales @ Mar 5 2007, 08:46 PM)
Question;
Is there a chart/table that lists the molecular weight of particles that sediment at different/certain RCF speeds?
ie. what G force sediments a 340 kDa protein?
or, at 100 000 G what molecules are we expecting to sediment?
Furthermore, at a high G like 100 000, what is the lightest molecular weight that will sediment?
Any help/ suggestions is greatly appreciated
Is there a chart/table that lists the molecular weight of particles that sediment at different/certain RCF speeds?
ie. what G force sediments a 340 kDa protein?
or, at 100 000 G what molecules are we expecting to sediment?
Furthermore, at a high G like 100 000, what is the lightest molecular weight that will sediment?
Any help/ suggestions is greatly appreciated
I think there is not a simple link between g force and sedimentation; each individual protein has a sedimentation coefficient (S)which can be experimentally determined or estimated by the method of Martin and Ames (1961); for estimation you need the reference of a similar (globular) protein knowing its Mw and S
-The Bearer-