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The various stage to start RT-PCR - HELP, please (Feb/17/2007 )

Hi,

In our laboratory we must establish RT-PCR, but there is not protocol and my memories of university are far blush.gif !!

therefore, if somebody can help me!!!!!!!

For example, what are the suppliers of such component?

Thanks

-boumbo_doc-

we buy kit from TAKARA and ROCHE. ROCHE gives better results

-ligation doesn't works-

QUOTE (ligation doesn @ Feb 19 2007, 12:17 AM)
we buy kit from TAKARA and ROCHE. ROCHE gives better results

hi ... we are using Roche light cycler 480 in our lab. its very reliable and has an easy to use software. recomended

-kklab-