making insert from miniprep DNA - (Feb/10/2007 )
sorry i misunderstood, Madrius wrote "endonucleases", but i thought it is endoglucanases=EG's 
-ligation doesn't works-
today i will try to make a vector from miniprep, i will let u know the result but till then pray for me
i am really get phoebia about ligation
-ligation doesn't works-