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Normalization-mirRNAs - (Jan/29/2007 )

Hi everybody, I used Real Time PCR for miRNA detection. I dont know how to do the normalization.I think that U6 RNA is the most suitable but how and if i can use rtPCR which are the primers and probe for U6RNA. Do you have any idea?
Tnanks you


HI, welcom to the biggest problem of miRNA RT_PCR detection. Yes, you can use U6, but U6 is not a you are never sure.Marco


yes but half life of U6 is above 100H...