Tn10 based Transposone mutagenesis - pLBT::mini-Tn10:lac:kan (Jan/22/2007 )
Does any one have any idea or experience working with the pLBT::mini-Tn10:lac:kan??
If you have/know: can you tell me which portion the plasmid is transferred in to the recepient chromosome??
Can anyone provide me the sequence of the pNK2804??
sorry lots of query
I don't really understand your question, but I think it is ment that Phage pLBT is used to make the coli strain (is mostly used to make a F'strain) with Tn10 (transposon 10, so resistent to tetracyclin, to make this F'(lac) and is also kanamycin resistant. Does this answer anything of your question?