pri-miRNA transcript - get the exact primary transcripts of miRNAs (Jan/17/2007 )
Does anyone kwow exact full transcripts of some miRNAs in fruit fly or other organisms? From the literature, I know some people failed to get the full transcript via RACE,RPA or Primer Extension. Any suggestions?
Then i also want to know whether any pri-miRNA will be capped and poly(A) tailed like messengerRNA after transcribed?
Best wishes to you!
I've read that many miRNA sequences are within introns, so I expect that those would not be capped and tailed but instead would be excised as splicing lariets. I do not know if ALL miRNA are intronic.
as far as i know, many microRNAs are located in the intergenic region and it seems that they have their own transcipt uints while intronic regional miRNA is more complicated.
I think most pri-miRNA should not have 5'-cap and 3'-polyA. If it does, why it is not transferred outside of the nucleus like mRNA? So ...
MicroRNAs and Their Regulatory Roles in Animals and Plants.
Have some good facts about it.