miRNA single or double stranded? - (Jan/10/2007 )
also I would recomend 'Molecular cell biology" 6th edition (Lodish et al, 2007) ---- it has a chapter on miRNA and siRNA very informative. and yes--- miRNA is doublestranded unless it is inside the RISC complex
I agree with the final conclusion that single strand microRNA is only happen after they entering into RISC. However, if this is the case, is it really possible to use IHC technique to stain miRs on the paraffin section? Especially the inactive miRNA?