why does ligase buffer precipitate? - is it ok just to vortex it?? (Jan/03/2007 )
Promega says it's DTT which precipitates in their Ligase buffer.
-exploresci-
well if DTT precipitates in the buffer efficiency will be decreased.
BSA precipitation does not realy matters. Just allow to warm longer and the bsa will dissolve.
-fred_33-
QUOTE (exploresci @ Jan 5 2007, 08:26 AM)
I think it's BSA which precipitates in ligase buffer when thawed, but this doesn't affect ligation efficiency.
this ligase buffer that i have from Nippon Gene doesnt contain BSA
. BSA is supplied seperatly in another tube. It contains Tris, MgCl2, DTT and ATP. If it is not DTT so maybe MgCl2 precipitates. 
-Kathy-