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Immunostaining of cytoplasmic proteins in nucleus, why? - (Jan/02/2007 )

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QUOTE (Mindo @ Jan 8 2007, 04:59 PM)
QUOTE (WAstate @ Jan 5 2007, 09:29 PM)
Are you using a retrieval step? Because sometimes over-retrieving the tissue causes faint "staining" in the nucleus.

we work with tissue cultures only, so I guess we do not perform retrieval step.

permeableisation (triton x) is a form of antigen retrieval (after a fashion) you could try reducing the time in triton x or use a protease based digestion.


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