Long Term Storage of DNA - I have a pool of cosmid library (Dec/31/2006 )
Hello Guys
Can anybody tell me whats the best way to store my cosmid library DNA for long times (years). I normally store at 4 degrees in TE buffer, but will it last for years.
Thanks for every help.
Happy New year.
-makosad05-
if you are clean and the cosmid is free from cellular endonucleases... then yeah, it will last years if not decades. (My supervisor has done this)
However, I know another PI, who has done this... and the cosmids were gone within a year.
So, there must be some element of skill involved.
For myself I keep my cosmids in TE at -80 and also in cells in glycerol at -80. It has lasted 1 year No idea if it will last decades though.
-perneseblue-
i routinely store DNA at -20. for long term stuff, -80.
V
-vetticus3-