To prepare fresh or not for sucrose gradient - Could it be stored longer? (Dec/25/2006 )
Hi,
I was wondering, within what time-frame do I have to use my layered sucrose gradient before it's not gradient anymore (e.g. due to diffusion)? Could the sucrose gradient be stored more than a day (or what's the maximum hours you've stored your gradient before using it)?
Many thanks.
-I love MSGs!-
i always use the gradients fresh. if you allow them to sit they will even out.
-mdfenko-
I have not tried, but store it at 4C should slow its diffusion. You can add some dye to the lower phase and see how it diffuses as time goes.
-genehunter-1-
Best to make the gradients fresh.
-scolix-
QUOTE (I love MSGs! @ Dec 26 2006, 09:39 AM)
Hi,
I was wondering, within what time-frame do I have to use my layered sucrose gradient before it's not gradient anymore (e.g. due to diffusion)? Could the sucrose gradient be stored more than a day (or what's the maximum hours you've stored your gradient before using it)?
Many thanks.
I was wondering, within what time-frame do I have to use my layered sucrose gradient before it's not gradient anymore (e.g. due to diffusion)? Could the sucrose gradient be stored more than a day (or what's the maximum hours you've stored your gradient before using it)?
Many thanks.
when pouring new gradients, add some phenol red to one of the two sucrose solutions; so you can visualize the gradient and get a feeling how long it will be stable by macroscopic inspection
-The Bearer-
Hey, thanks.
Guess fresh is the way to go then.
-I love MSGs!-