PAGE - (Mar/01/2003 )

Dear all,
At the moment I am running a polyacrylamide gel to separate DNA fragments of approximately 140bp, 90bp and 50bp. I am using 8% polyacrylamide in TAE buffer. I also tried TBE but I obtained basically the same results. I am running the gel for 2.5 hrs with a constant voltage of 200V. I am also using a cooling system to cool the buffer. The problem is that I am not getting well defined bands, they are sort of distorted. Can anyone suggest what can I do. Thanks.

email: dnalabmalta@yahoo.co.uk

is this a denaturing PAGE (i.e. with urea)? also, you should stay with TBE as it provides a better electrolytic buffer.