mutagenesis - tolerance (Dec/14/2006 )
hi Iwant to add a restricetion site to a sequence.
here the sequence
G GTT CGA TCT AGA ATG
I want to add an KPN1 site (GGTACC) and stay in frame.
I was thinking an aligo like :
...G GTA CCA CGA TCT AGA ATG....
my question is that does my oligo will work sinec it's not just a single nucleotide but 4 nucelotides change.
another thing is the CCA codon will provide a Proline. is it ok
what do you think guys?
previously i changed 4 nucleotide, its ok