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How long for ligations? - (Dec/11/2006 )

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i use Mighty mix from TAKARA . ligation condition is 16c for 30 min
i am also facing ligation problem sad.gif

-T. reesei-

I did the conventional method of ligation.

Put inside the fridge (about 4-6 celcius) for overnight ligation.

Works fine to me....


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