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Triton and electron microscopy - (Dec/03/2006 )

Hi everyone,
my antibody just works with triton, but I would like to visualize the antigen in electronmicroscopy. But therefore visible membranes are indispensably.... i think in this case triton is not necessary for permeabilization but for something else... has anybody an idea how to use triton without destroying the membranes, or does anybody know an easy antigen retrieval method without destroying membranes?
thanks
uschi

-uschifriedrich-

ok I understood..there might be no solution. very sad... cool.gif

-uschifriedrich-

QUOTE (uschifriedrich @ Dec 4 2006, 11:41 AM)
ok I understood..there might be no solution. very sad... cool.gif



Do you have cryo(ultrathin)section facility available to you? You dont need that once the samples are cut. You can also use hydrophilic resin to embed you sample then cut then stain. Either way Triton is not needed.

-genehunter-1-

Do you have cryo(ultrathin)section facility available to you? You dont need that once the samples are cut. You can also use hydrophilic resin to embed you sample then cut then stain. Either way Triton is not needed.
[/quote]

thanks for your answer, but the problem is that the staining( in immunfluorescence) is just working with triton... its not the permeabilization triton is needed for, it must be something else with the antigen accessibility. I dont´t know if its the fixation, but others tried it with different protocols, it just don´t work. And the studies with this antibody and electronmicroscopy are not very good, some used microwave antigen retrieval, or they just don´t talk about their method.
Maybe I just need another antibody....
Anyway I thought somebody has some speculations...

-uschifriedrich-