Is my plasmid contaminated by RNA? - (Dec/02/2006 )
QUOTE (I love MSGs! @ Dec 5 2006, 12:30 AM)
Hi,
Typo error: it's "without RE digest when using supercoiled DNA ladder for fast estimation".
DNA near the sink (or well), as dnafactory described, is gDNA. It's too large, therefore migrated slowly.
Do you mean plasmid conformations, e.g. supercoiled, nicked and linearized? Nope. It's too high to be linearized plasmid.
Typo error: it's "without RE digest when using supercoiled DNA ladder for fast estimation".
DNA near the sink (or well), as dnafactory described, is gDNA. It's too large, therefore migrated slowly.
QUOTE
can i assume it's dna fractions?
Do you mean plasmid conformations, e.g. supercoiled, nicked and linearized? Nope. It's too high to be linearized plasmid.
ok, i kinda figured it all out.
thanx a lot!!!!
i might pass the quiz thanx to u all
cheers
-biofreak06-
QUOTE (I love MSGs! @ Dec 5 2006, 12:30 AM)
Hi,
Typo error: it's "without RE digest when using supercoiled DNA ladder for fast estimation".
DNA near the sink (or well), as dnafactory described, is gDNA. It's too large, therefore migrated slowly.
Do you mean plasmid conformations, e.g. supercoiled, nicked and linearized? Nope. It's too high to be linearized plasmid.
Typo error: it's "without RE digest when using supercoiled DNA ladder for fast estimation".
DNA near the sink (or well), as dnafactory described, is gDNA. It's too large, therefore migrated slowly.
QUOTE
can i assume it's dna fractions?
Do you mean plasmid conformations, e.g. supercoiled, nicked and linearized? Nope. It's too high to be linearized plasmid.
just one more (i promise...)
I assumed by the 1st right lane that this is the biggest sample i got...
am i right?
thanx......
-biofreak06-
QUOTE (biofreak06 @ Dec 5 2006, 08:27 AM)
QUOTE (I love MSGs! @ Dec 5 2006, 12:30 AM)
Hi,
Typo error: it's "without RE digest when using supercoiled DNA ladder for fast estimation".
DNA near the sink (or well), as dnafactory described, is gDNA. It's too large, therefore migrated slowly.
QUOTE
can i assume it's dna fractions?
Do you mean plasmid conformations, e.g. supercoiled, nicked and linearized? Nope. It's too high to be linearized plasmid.
just one more (i promise...)
I assumed by the 1st right lane that this is the biggest sample i got...
am i right?
thanx......
-biofreak06-
Hi,
Can't tell if there's any plasmid or not in the first lane on the RIGHT.
-I love MSGs!-