20% chelex extraction method for forensic samples - (Nov/21/2006 )

Hi all,

Does anyone have a 20% chelex method for forensic samples (eg trace or degraded DNA) - or a way of getting around using Microcons to concentrate? I didn't realise how expensive they are!
Thanks.

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Hello,
I think the best method for extracting degraded DNA is the standard phenol chloroform method. Then you can concentrate with the microcon.

I haven't used the chelex in a while but I don't think it works as well.

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