f-stop - please explain (Nov/13/2006 )
I hope this is the correct place to post this.
My supervisor asked me today whether I used the same f-stop for my confocal and fluorescent microscopy images. I have no idea what he is talking about and neither does he since someone else asked him the question and he didn't know what they were talking about either. I did some Googling and honestly, I'm confused. It appears that f-stop is similar (or perhaps the same) as the aperature. In all my imaging I have never come across an f-stop setting so could someone please explain this. Thanks.
Yes, it's the aperture, and it's got something to do with photography. The f-stop (value) correlates to the amout of light taken up. Basically, a smaller f-value means the aperture is opened wider, i.e. more light is let through the lens to the film or sensor. So, f2.0 is brighter than f8.0, ok? (at the same exposition time)
In addition, it's got something to do with depth of field, that is the depth you'll be able to show sharp in the picture (at f2.0 much less depth is "sharp" while 8.0 allows much more depth to be shown sharp at the same time). If you want more details on this, you should visit some photography forums or sites.
So, to make it short, I think your boss asked you if both picture were take under the same settings, which you should have done, just to make brightness levels comparable.
Mike
In addition, it's got something to do with depth of field, that is the depth you'll be able to show sharp in the picture (at f2.0 much less depth is "sharp" while 8.0 allows much more depth to be shown sharp at the same time). If you want more details on this, you should visit some photography forums or sites.
So, to make it short, I think your boss asked you if both picture were take under the same settings, which you should have done, just to make brightness levels comparable.
Mike
Thanks. I kind of got that from a few sites I read. So would this setting be considered similar to the pinhole setting on a confocal microscope, which also regulates the amount of light as well as the field of depth?
Yes, it's the aperture, and it's got something to do with photography. The f-stop (value) correlates to the amout of light taken up. Basically, a smaller f-value means the aperture is opened wider, i.e. more light is let through the lens to the film or sensor. So, f2.0 is brighter than f8.0, ok? (at the same exposition time)
In addition, it's got something to do with depth of field, that is the depth you'll be able to show sharp in the picture (at f2.0 much less depth is "sharp" while 8.0 allows much more depth to be shown sharp at the same time). If you want more details on this, you should visit some photography forums or sites.
So, to make it short, I think your boss asked you if both picture were take under the same settings, which you should have done, just to make brightness levels comparable.
Mike
Thanks. I kind of got that from a few sites I read. So would this setting be considered similar to the pinhole setting on a confocal microscope, which also regulates the amount of light as well as the field of depth?
Yep
My supervisor asked me today whether I used the same f-stop for my confocal and fluorescent microscopy images. I have no idea what he is talking about and neither does he since someone else asked him the question and he didn't know what they were talking about either. I did some Googling and honestly, I'm confused. It appears that f-stop is similar (or perhaps the same) as the aperature. In all my imaging I have never come across an f-stop setting so could someone please explain this. Thanks.
The f-stop in photography is a combination of the speed of the film, the size of the appature and the lengh of time of exposure.
it means you can keep the stop the same, increase the amount of exposure and compensate by reducing the appature or using a slower speed of film - you find it on slr camers's.
essentially the stop is the final picture produced using various variables
it means you can keep the stop the same, increase the amount of exposure and compensate by reducing the appature or using a slower speed of film - you find it on slr camers's.
essentially the stop is the final picture produced using various variables
actually, the f-stop is only the aperature size.
it means you can keep the stop the same, increase the amount of exposure and compensate by reducing the appature or using a slower speed of film - you find it on slr camers's.
essentially the stop is the final picture produced using various variables
actually, the f-stop is only the aperature size.
That's true, the F-stop is only the aperture size. Just look at any 35mm SLR lens. He obviously doesn't know his photography, it has nothing to do with film speed.
it means you can keep the stop the same, increase the amount of exposure and compensate by reducing the appature or using a slower speed of film - you find it on slr camers's.
essentially the stop is the final picture produced using various variables
actually, the f-stop is only the aperature size.
That's true, the F-stop is only the aperture size. Just look at any 35mm SLR lens. He obviously doesn't know his photography, it has nothing to do with film speed.
I got my F-stop mixed up with my stop (a common term in both photography and dark room chemistry)
- not to be confused with the stop bath i might add.