DEPC treatment, EDTA? - (Oct/25/2006 )

Hello,

can you please tell me if it is possible to treat EDTA with DEPC? Is there a list anywhere were you can look up which substances can be treatet and which not? I always read that some substances like tris cannot be treatet, but I am not good enough at chemistry to deside for myself what else cannot be tratet.

So thank you for any help!

Bianca

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hi
DEPC destroys all amines
as EDTA contains amine residue it can't be DEPC treated but should be prepared with pretreated water

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I think DEPC only reacts with the primary and secondary amines, but not with the tertiary amines. EDTA has only the tertiary amines in its structure. So I think it would be safe to treat EDTA solution with 0.1% DEPC before autoclaving.

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well maniatis doesn't seem to be with you

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Please check out this site:

http://www.biosupplynet.com/cfdocs/protoco...-aug-2006-2.cfm

I read: "Make up Tris-Cl (pH 7.6) from a fresh bottle in autoclaved, DEPC-treated H2O. Prepare NaCl and EDTA in 0.1% DEPC in H2O. Store for at least 12 hours at 37°C and autoclave the mixture for 15 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. "

It seems that their protocol is excerpted from the newest "Molecular Cloning" by Sambrook, although Maniatis is no longer in the author list.

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