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7AAD and PI - (Oct/24/2006 )

Dear all
I'm performing FACs analysis for cell cycle study. For determining DNA content i can use PI or 7AAD. But what is the difference between PI and 7AAD? Have i to treat samples whit DNasi or RNasi before performing facs analysis?
Thank in advance! all the best.


you cannot block all polymerases by actinomycine D.
At least shoul you use alpha amanitine too.