Why is DMSO used for error-prone PCRs? - (Oct/20/2006 )
Could someone tell me what for is the add of DMSO for error-prone PCRs?
DMSO interferes with hydrogen binds between two strands of primer and template. This way it overcomes the need to increase temperature of denturation. Think this way: DMSO:PCR::SDS:Western Analysis
I read somewhere saying that DMSO is the inhibitor for transformation. Is it true? But I used DMSO to dissolve XGal instead of formamide. I guess with that small amount or diluted DMSO, hopefully it wouldnt affect my transformation.
Sorry, ale to use your thread. =)
My understanding was that DMSO breaks up secondary structures. So if you have a GC rich template, it can aid in amplification by denaturing any pin structures that prevent extension. Betaine does the same thing. I have found that a long initial denaturation of 10 minutes (prior to addition of polymerase) can also help by ensuring your template is completely denatured.
Yes, with CG rich templates facilitates denaturation step allowing the Polymerase to extend, but in DNA shuffling is what I don t get what is this for