Why is DMSO used for error-prone PCRs? - (Oct/20/2006 )

Hi everyone

Could someone tell me what for is the add of DMSO for error-prone PCRs?
Thanks

DMSO interferes with hydrogen binds between two strands of primer and template. This way it overcomes the need to increase temperature of denturation. Think this way: DMSO:PCR::SDS:Western Analysis

I read somewhere saying that DMSO is the inhibitor for transformation. Is it true? But I used DMSO to dissolve XGal instead of formamide. I guess with that small amount or diluted DMSO, hopefully it wouldnt affect my transformation.

Sorry, ale to use your thread. =)

My understanding was that DMSO breaks up secondary structures. So if you have a GC rich template, it can aid in amplification by denaturing any pin structures that prevent extension. Betaine does the same thing. I have found that a long initial denaturation of 10 minutes (prior to addition of polymerase) can also help by ensuring your template is completely denatured.