PFGE equipment - (Oct/20/2006 )

Morning all,
We're setting up BAC recombination in our lab at present and require a pulse field gel electrophoresis system to separate large fragments of DNA. I was just wondering if I could get any advice on what supplier/system to use? We only need standard conditions (i.e angle of 120 degrees) so the Biorad DR2 system has been suggested. I was just wondering if anyone on here had any good recommendations?
Thanks

-aadamson81-

I myself used the Rotaphor system from Biometra quite sucessfully in the past. It's a rotating field PFGE machine. Not that I needed the "extra" angles it can provide, as I also did only BAC analyses, but the machine was already there in the lab, so I used it. Worked quite well for me....

Mike

-jadefalcon-

QUOTE (jadefalcon @ Oct 20 2006, 03:12 PM)

Thanks mate, it's been between that and the biorad. Think i'll try and play the reps off against each other and see who offers me the best price!

-aadamson81-

QUOTE (aadamson81 @ Oct 20 2006, 03:25 AM)

We used CHEF from BioRad. It worked well and mapper was friendly:)
Note: Some people complain about the longevity of the electrodes.

-Liliana-

QUOTE (Liliana @ Oct 25 2006, 04:16 AM)

QUOTE (aadamson81 @ Oct 20 2006, 03:25 AM)

We used CHEF from BioRad. It worked well and mapper was friendly:)
Note: Some people complain about the longevity of the electrodes.

true, they can be easily damaged

-ahmad193-

Hi, We also use the Chef DR-II from Biorad and have had no problems with the electrodes. Maybe we are lucky!

-Techqueen-