Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

Problem with EGFP in translation study - (Oct/10/2006 )

Pages: Previous 1 2 

Thank You

I obtained only specific bands and no any signal in the untransfected cells, therefore my FLAG antobody works well.
I think, the problem is in transfection/translation efficiency. i will try first to increase this efficiency. If it will not work, maybe i will try FACS
L

-luukasz4-

could you co-transfect fluorescent actin or anything like this as control for your transfection efficiency?

-Lab Dance-

I havesolved the problem - it was the problem of the microscope.
Thank You for help
L

-luukasz4-

Pages: Previous 1 2