Problem with EGFP in translation study - (Oct/10/2006 )
I obtained only specific bands and no any signal in the untransfected cells, therefore my FLAG antobody works well.
I think, the problem is in transfection/translation efficiency. i will try first to increase this efficiency. If it will not work, maybe i will try FACS
could you co-transfect fluorescent actin or anything like this as control for your transfection efficiency?
I havesolved the problem - it was the problem of the microscope.
Thank You for help