Dialisis of samples for electroporation - (Sep/30/2006 )

Hello

One question:
How do you dialize your DNA samples for electroporation?
I mean, do you use a membrane dialisis. Which one (brand)?
Do someone know if nitrocellulose membranes for western blot works for this?

THANKS

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EtOH precipitation is fast and simple. If you insist to use dialysis, any regular dialysis tubing would do, if you are not dealing with synthetic oligos.

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there should be some more information...electroporation is used f.i. to get samples (DNA, proteins) out of gel or membrane pieces into solution by electric current; therefore use your running buffer for DNA agarose gels; dialysis tubes or tubes with filters (for special electroporation apparatus) can be used; in solution of dialysate, DNA can be precipitated as suggested by genehunter-1;

nitrocellulose can be used for electro transfer (Southern blot) of DNA but this porcedure is not known as electroporation

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The easiest way, in my experience, is to do drop dialysis on the DNA. See an NEB description of the techniques and materials here:
http://www.neb.com/nebecomm/techBulletinFi...OP_Dialysis.pdf

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Hi,

We use dialisis membranes from Millipore. We just put some ddH2O in a petri dish and one dialisis disc floating. In the top of the disc we drop the ligation and incubate for half an hour. It works !!! The electroporation works much better after.

Regards,

Antonio

PS: If you arei ntereted I can give you exactly the catalog number

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That's what we do too but we have ran out of them. I saw that they are nitrocellulose filters which is the same material of membranes for western blotting. so I wondered if can I use it instead of millipore discs.

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nitrocellulose will bind DNA if it is not really good blocked; dialysis filter membrane are characterized by low binding capacity and form stable in buffer; I would not use NC

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