splice variants and knock down - (Sep/27/2006 )
my targeting gene has 3 splice variants. it's long and overlapping each other.
will it affect my knock down?
well if the strategy is siRNA, if the sequence is shared by the 3 normally the 3 should be affected (if the siRNA itself is active).
If the siRNA targets a unique sequence, the only mRNA containing the sequence would be knocked down
Depends on ur siRNA sequence. if the sequence is there in all of the 3 variants, and u have good sequence to knock it, then it should work.