need some help in secondary antibody - what does it means when .... (Sep/27/2006 )
Hi,, I am trying to understand the product sheet of a HRP-secondary antibody. What does it means when they have been absorbed against the serum proteins of a large number of species to virtually eliminate background and non-specific staining. I understand the purpose of doing that, but I have no clue in what they mean by absorbed against serum....???
Can anyone help me here?
Thank you very much for your comments.
My understanding is that they run the secondary antibody over columns that will bind each different species' serum proteins that you don't want. If you get a goat anti rabbit secondary, they have run it over columns that will bind the mouse, horse, etc. serum IgG proteins that naturally exist in the goat's serum.
Ohhh, is like a purificatin process to assure that the antibody will bind to the species's first antibody that you are using.