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Protocol for irradiated APC from spleen - (Sep/27/2006 )

I was wondering how to prepare irradited APC, Iwant to use these for costimulation to differntiate cells to Th1 lineage. Also how does CD90 depletion affect costimulation

Thanks In advance


I don't know if it's a general procedure, we used 3000 rad to irradiate APC for lymphocyte stimulation.