Question about dialyse - (Sep/25/2006 )

Someone would like to know how to dialyse a protain of 13000 Da!! He would like to remove NOG
What to use?

Help him !!!!

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What is the weight of NOG (what is NOG)?
Because there are dialyses membranes with a broad variety of cut-off values. So either choose one that you remove the NOG and keep the protein inside (a cut-off just below 13 kDA) or remove your protein from the membrane and keep the rest in (cut-off just over 13kDa).
Dialyse against the solution in which the protein is dissolved (without salts).

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centricon columns may be the better way for him to purify the protein and concentrate it in same way.

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Dear Margo 33,


Agree with Aspergillie entirely. Practically get dialysis tubing either dry or already soaked in buffer. I normally dialyse a large volume of FCS to remove Arginine. This is done by taking 100ml of FCS and dialysing against 10L of PBS. This is repeated 3 times, replacing the PBS every 24hours. The FCS supports cell growth, and in my assay Arginine is completely removed.

Hope this is useful

Regards

Rhombus

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