Rag KO mice - expert needed (Sep/19/2006 )

Hi everyone!

Can anyone tell me... Do rag -/- mice (witch don't have B or T cells) have NK cells?

Thanx.

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Hi D,

They do have NK cells but not NK T cells. You may want to read the following works:

Reading PC, Whitney PG, Barr DP, Smyth MJ, Brooks AG. 2006. NK cells contribute to the early clearance of HSV-1 from the lung but cannot control replication in the central nervous system following intranasal infection. Eur. J. Immunol., 36(4):897-905.

King A, Gardner L, Sharkey A, Loke YW. 1998. Expression of CD3 epsilon, CD3 zeta, and RAG-1/RAG-2 in decidual CD56+ NK cells. Cell. Immunol., 183(2):99-105.

Taniguchi M, Koseki H, Tokuhisa T, Masuda K, Sato H, Kondo E, Kawano T, Cui J,
Perkes A, Koyasu S, Makino Y. 1996. Essential requirement of an invariant V alpha 14 T cell antigen receptor expression in the development of natural killer T cells. Proc. Natl. Acad. Sci. U S A., 93(20):11025-8.

Gerardo

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Hi Gerardo,
I'd like to ask a follow up question to BioPe's question. I was guessing that they might have NK cells but I've read that NK T cells have a semi/ completely invariant TCR? Does this need genetic recombination to be expressed and is it different in different species?

Thanks,
Ceri

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Hello Ceri.

I am not sure, but I think the last arthicle that Gerardo indicated partially answers to your question.

Thanks!

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