Qn on Antibody - Technical question (Sep/06/2006 )

Hi,

I am looking for antibodies to detect CD19 (Anti-Leu 12) and CD71 (anti-CD71)on cells.
I wonder if anyone can explain what anti-leu 12 and anti-CD71 means?

Thank you.

Light my way please....

-light_my_way-

"Anti-X" means the antibody is raised against the antigen X, so will bind to X. In your case CD71 and CD19. I'm guessing the Leu 12 may be a historical/older name for CD19 before the CD classification came in. Surface antigens on leukocytes were defined on the basis of antibodies raised against them. So Leu 12 could be Leukocyte antigen 12 or something like that or it could just be the antibody clone name to distiguish between different antibodies against the same antigen.

All the best,
Ceri

-Ceri-

QUOTE (Ceri @ Sep 7 2006, 04:28 AM)

I'm guessing the Leu 12 may be a historical/older name for CD19 before the CD classification came in. Surface antigens on leukocytes were defined on the basis of antibodies raised against them. So Leu 12 could be Leukocyte antigen 12 or something like that or it could just be the antibody clone name to distiguish between different antibodies against the same antigen.

or it could be directed toward the neighborhood of leucine at residue 12 of cd19.

-mdfenko-