Tm of probe - (Sep/03/2006 )

how o calculate the Tm of probe to be used insouthern or northern

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I think it iswith the farmula


Tm = 4 (G + C ) + 2( A + T )
where as G, C, A, T are total nucleotides in probe

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And calculating this gets you down or is too much of a bother, there are oligocalculators on the Net. Do a google search to find them.

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i guess the formula does not fits into the calculation of 637 bp probe

thnaks

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