Counting methods - dinoflagellates (Sep/02/2006 )

can anyone give some methods that how can i count the cells?

I grow the cells in the 500ml conical flask and i want to know the number of cells in the 500ml flask,
thanks

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Dont know much about dinoflagellates but can suggest u use a haemocytometer or any flow cytometer.

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Try counting chambers eg. Abbe-Zeiss counting cell chamber. They look like similar to normal microscope slides and have a big cover slip. They have a defined volume (made from the slip) were you then apply your well mixed sample. You count cells in the a part of the grids (the surface is segmented by a grid) under a microscope and can calculate total cell number. It is normally used for blood cells etc.

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There is a book "Manual on harmful marine microalgae", one of the chapter has mentioned how to do the cell counting of dinoflagellates. This is a really good book for people working on dinoflagellate research

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