70% Ethanol... - (Aug/22/2006 )
I did a Phenol/chloroform extraction today. But unfortunatly, I added 70% ethanol instead of 100% ethanol when doing ethanol precipitation. What's the worst consequence can be, and what can I do to make it up?
Worst: you'll never recover your DNA.
If you have space left add 100% EtOH until you get a final conc of 70%. If not then transfer to a bigger tube add 100% EtOH to 70% final volume and then split the mixture into smaller tubes to spin down.
And will it still lower the yield to a great extent in this way?
Do you mean the conc of Ethanol? Then how about the conc of NaAc?...
I really worry about the DNA now...
If you can somehow get the final volume of EtOH in your sample up to 70% (by adding 100% EtOH) you should be able to retrieve most of your DNA.
As for NaOAc, add 1/10th volume of 3 M.
Thanks a lot!! Hope I won't be punished too hard.