Any effect of different culture dishe size on cell? - (Aug/20/2006 )

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QUOTE (genehunter-1 @ Sep 15 2006, 07:35 PM)

A correction:

I think the number listed for 100 mm dish is wrong. According to my measurement, the diameter for 100 mm dish is only 8.5 cm, not 10 cm the name indicated. This translates to a surface area of ~ 5671 mm^2, or ~75% of the surface of listed. So multiply 0.75 to the numbers for 100 mm dish everyone!!!

I become suspicious when I saw the numbers of 100 mm dish and T75 flask. We all know a T75 is bigger than 100 mm dish, right?

If the culture dishes are not 100mm, then why are they still called "100mm culture dish"?

-Minnie Mouse-

QUOTE (Minnie Mouse @ Sep 17 2006, 07:36 PM)

If the culture dishes are not 100mm, then why are they still called "100mm culture dish"?

I think the size is based on the top cover of the 10cm dish, wherease the base of the dish is the culturing area which is smaller than the cover.

-scolix-

i think it depends on the manufacturer...
you can request 10, 9 or even 11 cm dishes, and all are commonly named "10 cm" but it is not always exact!
check the product sheet
Seb

-tryptofan-

the pdf is very useful, thanks a lot

-T. reesei-

Depending on your cell type there could be some non-density related issues. In a smaller well there is less space to move around and the cells will find the edges quicker which will have some effects on cell orientation...

D

-DLY-

QUOTE (pfmdr @ Aug 20 2006, 07:58 PM)

hi

can anybudy tell me

is there any effect of using different size of dishes for culturing

The size of dish does matter at times. You need to use higher cell density for larger dishes. This is good if you want more cells (for protein extraction, or other reasons). However, dish size does shows much difference if you are measuring the concentration of a biochemical in culture supernatant.

Regarding the pdf sent, its a very good one. But, regarding 24-well plates, i have been using the cells at densities as high as 1-5 million cells/well (i ml media per well) for abt 4-6 days without any notable problem.

-rpsingh-

there is a stupid question I d like to ask: do the densities of the cell mean per cm2 or ml?

-subberry-

QUOTE (subberry @ Jun 1 2007, 12:30 AM)

there is a stupid question I d like to ask: do the densities of the cell mean per cm2 or ml?

Not a stupid question at all, both are correct but they deal with the 2 different ways of growing cells. For example:-

J774/RAW 267.4 cells can grow in SUSPENSION culture. Their density should not exceed 750,000 cells/ml.

If you grow the same cells on plastic i.e. as ADHERENT cells then the density of cells can be expressed as cells/cm2.

Hope this clears any confusion. If you do not ask questions then you will never learn.

Regards

Rhombus

-Rhombus-

QUOTE (Rhombus @ Jun 1 2007, 04:30 PM)

QUOTE (subberry @ Jun 1 2007, 12:30 AM)

there is a stupid question I d like to ask: do the densities of the cell mean per cm2 or ml?

Thank you for your warmhearted expalination. when I look in the table attached in this topic(useful numbers for cell culture), I wander the cell number used in this table means by per cm2, right?

-subberry-

QUOTE (DLY @ May 16 2007, 03:23 PM)

Depending on your cell type there could be some non-density related issues. In a smaller well there is less space to move around

yep.. i agree with that

QUOTE (DLY @ May 16 2007, 03:23 PM)

and the cells will find the edges quicker which will have some effects on cell orientation...

D

but, i dont really understand what this statement means... is there anyone, maybe DLY, could explain ? just for curiosity sake... thanks a lot

-delimartin-

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