Silver stainging after coomassie? - SDS-PAGE (Aug/15/2006 )
Hello,
I stained my gel with coomassie, but the expecting bands are invisiable... I want to stain it again with silver. Can I just destaine my gel and then fix and do a silver staining. Will these two process conflict? 
-yeping-
You can stain your gel with silver after coomassie. I have done this with the Bio-Rad SilverStain Plus, was no problem. They just say you should destain the gel with 40% Methanol, 10% acidic acid. And even though it did not destain very much, my silver stain afterwards was very nice. Just try it!
-biomaus-
QUOTE (biomaus @ Aug 15 2006, 02:28 PM)
You can stain your gel with silver after coomassie. I have done this with the Bio-Rad SilverStain Plus, was no problem. They just say you should destain the gel with 40% Methanol, 10% acidic acid. And even though it did not destain very much, my silver stain afterwards was very nice. Just try it!
cool! how did u think that up?
No reference to technique............
-Microbiologist-
biomaus wanted to say acetic acid
-Missele-
Right, Missele, sorry, very early 
-biomaus-
Thank you all for the answer and discussion 
Now, I can do it with confidence.
-yeping-