help with concentration calculation - Calculation (Aug/03/2006 )
Minnie Mouse,
try typing in the url directly onto the address bar but here I've tried again.
http://www.wmtc.org.uk/
Cheers
-paraboxa-
QUOTE (paraboxa @ Aug 15 2006, 01:06 AM)
Minnie Mouse,
try typing in the url directly onto the address bar but here I've tried again.
http://www.wmtc.org.uk/
Cheers
try typing in the url directly onto the address bar but here I've tried again.
http://www.wmtc.org.uk/
Cheers
Thank you paraboxa
-Minnie Mouse-
QUOTE (spanishflower @ Aug 4 2006, 02:55 AM)
goodmorning all
how are u all today?
i have a serious problem in calculation.... that i am toooooo bad in it, i need an advice.
i checked some of my old lectures abouts solutions and its preparation.
what shall i do?
how are u all today?
i have a serious problem in calculation.... that i am toooooo bad in it, i need an advice.
i checked some of my old lectures abouts solutions and its preparation.
what shall i do?
Hi Spanishflower,
The sites for doing calculations are good but I wouldn't use them myself. I will agree with others who said that take paper-pencil and spend some time practicing it. Believe me calculations for molar, mole, normal solutions are very simple once you practice doing it yourself. Calculations for ED50, IC50, % inhibition, % induction too are simple using microsoft excell.
Hope it helps.
Pravin Mahajan
-exploresci-
[font=Arial Black][size=7]Thanks for good link so much It pretty good for my experiment
-Ladyaek-
QUOTE (paraboxa @ Aug 15 2006, 04:06 AM)
Minnie Mouse,
try typing in the url directly onto the address bar but here I've tried again.
http://www.wmtc.org.uk/
Cheers
try typing in the url directly onto the address bar but here I've tried again.
http://www.wmtc.org.uk/
Cheers
Paraboxa,
I cannot find 'past papers' or the link to the calculations from Deacon from this link. Can you give a direct link to them? Like others, I am practicing as much as I can on dilutions & biochemical calculations.
-nk111-
QUOTE (genehunter-1 @ Aug 11 2006, 03:32 PM)
it will be 2000 times of the number that you derived from the curve.
Just seeking a clarification on the MTT assay for calculating the IC50. having obtained the sigmoid shaped log curve using cell viability (%) and drug concentration on the y and x axes, respectively, I extrapolate from 50% cell viability across and down to get the concentration (IC50). this value that I get, do I need to do any further calculations with it? Or is it the final answer for the IC50? it is a log scale on the X-axis so I am wondering if i need to change back the answer from log form?????????
Thanks
-mrcellline-
QUOTE (mrcellline @ Apr 2 2008, 12:38 AM)
QUOTE (genehunter-1 @ Aug 11 2006, 03:32 PM)
it will be 2000 times of the number that you derived from the curve.
Just seeking a clarification on the MTT assay for calculating the IC50. having obtained the sigmoid shaped log curve using cell viability (%) and drug concentration on the y and x axes, respectively, I extrapolate from 50% cell viability across and down to get the concentration (IC50). this value that I get, do I need to do any further calculations with it? Or is it the final answer for the IC50? it is a log scale on the X-axis so I am wondering if i need to change back the answer from log form?????????
Thanks
IC50s are given as concentrations, so yes, you need to go from log form to normal notation. I assume that you're plugging a y-value into an equation and getting x, the IC50, out, correct?
-TheSquire-