Beta-Mercaptaethanol - (Aug/05/2006 )

I have to use beta-mercaptaethanol in concentation of 20mM but i have 2-mercaptaethanol solution. Its in liquid form . I am fix how can i calculate how much i have to use for 20mM.
On bottle its written Mr=78.01.\
please suggest me.
regards

-samita-

Hi.
The sigma Beta Mercaptoethanol which we use in our lab is always 14.3M. Which company Beta ME do you use? You can check in that company's website.

-Calvin*-

Do you know the density of your 2-mercaptoethanol solution? Then you should be able to easily calculate the molarity.
The one in our lab was 14,3M, too.

-speedy-

Hi samita
can u tell me from which company u r purchasing 2-mercaptoethanol, we r using 2-mercaptoethanol from SIGMA whose molecular weight is 78.13, molarity is 14.3 M and density1.114g/ml. Its better to see the catalogue of manufacturer.
good luck
prabhanshu tripathi
IGIB (CSIR), Delhi,INDIA
Mobile-9868613020

-prabhanshu-

commercial solutions are generally at 14.3 M

-fred_33-

mine is from Bio-Rad, it's 14.2 M

-Missele-