DGGE - (Aug/02/2006 )

Hi,

I run DGGE today for 18s rDNA of mix fungi samples. I use 8% gel and the denauring gradient from 17-43%. The gel was run at 180 v, 58 C for 18 hours. But the sample DNA just migrate less than 1 cm . The mix DNA sample (1600bp) is not seperated. I may need to change tthe running condition to get mix DNA seperated. who can tell me how to adjust it and seperate them.

Thanks!!

i think you should try it again with the samples that have already worked. if it works, there would be no problem with the stock solutions, otherwise you might need to change that.

cheers!

Hi,

I run DGGE today for 18s rDNA of mix fungi samples. I use 8% gel and the denauring gradient from 17-43%. The gel was run at 180 v, 58 C for 18 hours. But the sample DNA just migrate less than 1 cm . The mix DNA sample (1600bp) is not seperated. I may need to change tthe running condition to get mix DNA seperated. who can tell me how to adjust it and seperate them.

Thanks!!

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hello,

Are you sure that fragments of 1600 pb can be loaded and separed under DGGE?
Are your DNA amplifyed fragments with a GC-clamp primers??