Protocol Online logo
Top : Forum Archives: : General Lab Techniques

Ethidiom bromide spills on my foot - (Jul/30/2006 )

Pages: Previous 1 2 

I think off is better

I have one of those ID's on a leash, that you hang around your neck. at my height, when I lean over the bench to grow bugs or set up a pcr, the thing would drag right through if I actually wore it on my neck

the question is, do these guys wear the same tie when they go to the lunch room and eat????

-aimikins-

Working in a hospital I see doctors going out to lunhc wearing their labcoats and having their stethoscopes haning out their pockets.

Once I a colleague of mine in another lab had to give me a tube from a BSL3 lab, so he left his office, put on his labcoat, went to the BSL3 lab, took his labcoat of right there, and then (of course) took his personal BSL3 labcoat and all that. So this person just wore his labcoat to walk down the corridor which is strictly forbidden... Happens all the time though, people aren't strict enough about biosafety regulations and all that.

-vairus-

QUOTE (aimikins @ Aug 1 2006, 08:17 AM)
I think off is better

I have one of those ID's on a leash, that you hang around your neck. at my height, when I lean over the bench to grow bugs or set up a pcr, the thing would drag right through if I actually wore it on my neck

the question is, do these guys wear the same tie when they go to the lunch room and eat????


You may put the ID card on your back when doing experiment, so that the card will never have to touch the bench. smile.gif

-Minnie Mouse-

For ID cards the simple solution is to by ski pass holder with a retractable cord. These are dead cheap and you clip them to your belt. The cord is about 1 metre long and coils into the mechanism so it's neat and tidy.

We use these around our facility. No danger of contamination and we can extend the cord to reach all the card readers.

-Doc_Martin-

I'm just curious... what will detergent do if used to remove EtBr?

QUOTE (akhshik @ Jul 30 2006, 10:41 PM)
QUOTE (pcrman @ Jul 30 2006, 03:03 AM)

Your foot should be OK because you have cleaned it with plenty of water. Although EB is a carcinogen I don't think contacting once will cause cancer.



you have to cut your foot befor EtBr systematically transfer to your whole body wink.gif

dont worry nothing special is going to happen to your foot. but remember never use detergent to remove EtBr.

-xtrios-

QUOTE (xtrios @ Aug 23 2006, 10:48 AM)
I'm just curious... what will detergent do if used to remove EtBr?

QUOTE (akhshik @ Jul 30 2006, 10:41 PM)

QUOTE (pcrman @ Jul 30 2006, 03:03 AM)

Your foot should be OK because you have cleaned it with plenty of water. Although EB is a carcinogen I don't think contacting once will cause cancer.



you have to cut your foot befor EtBr systematically transfer to your whole body wink.gif

dont worry nothing special is going to happen to your foot. but remember never use detergent to remove EtBr.





i dont know why but i read that in case of eye contact wash with cold water for 15 minutes and in case of skin contact wash with water and soap so i dont know why not to use detergent.

-spanishflower-

QUOTE (pcrman @ Jul 30 2006, 06:03 AM)
Although EB is a carcinogen...


QUOTE (Astilius @ Jul 31 2006, 06:14 AM)
Ethidium bromide is a strong carcinogen - one exposure is not safe....


Where does this stuff come from? I guess it's because we all know that EtBr reacts with DNA, and thus we all assume it is a carcinogen. There is very little data to support this. So little in fact that it's not even classified as a carcinogen by the US Governmnet.

See, for example, here, with reference to part 11 (Toxicological Information), which notes that it is neither a known or anticipated carcinogen with regards to NTP (the National Toxicology Program), and has no IARC (International Agency for Research on Cancer) listing.

See also, for example, here, where it is pointed out that:

QUOTE
Ethidium Bromide is a potent mutagen and moderately toxic after an acute exposure and although there is not sufficient evidence at this time to officially class Ethidium Bromide as a human carcinogen or teratogen it should, nevertheless, be treated as a possible carcinogen and teratogen.


It is clearly toxic, and thus should be handled carefully, but calling it a carcinogen (or worse a "strong carcinogen") is dubious at best. It's been in daily use in countless labs for decades around the world -- is there a single documented case of cancer caused by EtBr?

Relax, your foot will be fine...

-HomeBrew-

QUOTE (akhshik @ Jul 30 2006, 07:41 AM)
QUOTE (pcrman @ Jul 30 2006, 03:03 AM)

Your foot should be OK because you have cleaned it with plenty of water. Although EB is a carcinogen I don't think contacting once will cause cancer.



you have to cut your foot befor EtBr systematically transfer to your whole body wink.gif

dont worry nothing special is going to happen to your foot. but remember never use detergent to remove EtBr.

-Glycome-

QUOTE (Glycome @ Aug 22 2006, 09:18 PM)
QUOTE (akhshik @ Jul 30 2006, 07:41 AM)

QUOTE (pcrman @ Jul 30 2006, 03:03 AM)

Your foot should be OK because you have cleaned it with plenty of water. Although EB is a carcinogen I don't think contacting once will cause cancer.



you have to cut your foot befor EtBr systematically transfer to your whole body wink.gif

dont worry nothing special is going to happen to your foot. but remember never use detergent to remove EtBr.


Do not worry.You leg is exposure to EtBr for a shrt time.I think next time you should take care wile working in lab,follow the safty procedures.

-Glycome-

i read its mutagenic not carcinogenic.

-spanishflower-

Pages: Previous 1 2