PFGE: how do i improve band clarity? - (Jul/26/2006 )
I am doing PFGE to investigate heterogeneity among a population of GBS. However, when I run the gel, the bands are slanted and blurred. The marker appears clearer but my samples have alot of background staining. I tried increasing the number of washes to 5. THis has improved things slightly but any other suggestions on changes I could make?
-raeche-
QUOTE (raeche @ Jul 26 2006, 08:22 PM)
I am doing PFGE to investigate heterogeneity among a population of GBS. However, when I run the gel, the bands are slanted and blurred. The marker appears clearer but my samples have alot of background staining. I tried increasing the number of washes to 5. THis has improved things slightly but any other suggestions on changes I could make?
I'm not a PFGE expert, but in my case, when cooler did't work (the temperature went up to 27C), I got blurred bands. Check cooler and circulation.
-yja97-