how to extract nuclear protein - help (Jul/23/2006 )

hi everyone:
my interest protein are p-erk and p-p38.they're both in nuclear.
can anyone tell me how to extract the protein in nuclear?
Thanks!

-july-

Usually with RIPA or NP-40 or Urea, you can extract nuclear and cytoplasmic proteins. Do you mean that you want to extract nuclear only, getting rid of the cytoplasmic proteins? If so, let me know and I'll send you the protocol as soon as I go to the lab

-dnafactory-

QUOTE (dnafactory @ Jul 23 2006, 06:09 PM)

I want to extract both nuclear and cytoplasmic proteins.
Can you tell how to get NaF solution?
Thank you!

-july-

QUOTE (july @ Jul 23 2006, 04:43 PM)

QUOTE (dnafactory @ Jul 23 2006, 06:09 PM)

I want to extract both nuclear and cytoplasmic proteins.
Can you tell how to get NaF solution?
Thank you!

You should dissolve the powder in water, is this what you want to know? Here is the link from Sigma where you can find the info you need: http://www.sigmaaldrich.com/catalog/search...tail/SIAL/S7920

-dnafactory-

i've posted my protocols for protein preparation here. They're fine for your goal.
You can add NaF and/or sodium orthivanadate is you matter.

-fred_33-

QUOTE (dnafactory @ Jul 23 2006, 03:09 AM)

hi ,

Could you provide me a copy of protocol for only extracting nuclear proteins!

Thanks very much!

-DNA-

QUOTE (DNA @ Jul 24 2006, 06:19 PM)

QUOTE (dnafactory @ Jul 23 2006, 03:09 AM)

hi ,

Could you provide me a copy of protocol for only extracting nuclear proteins!

Thanks very much!

Check your PM

-dnafactory-

QUOTE (fred_33 @ Jul 24 2006, 11:53 PM)

i've posted my protocols for protein preparation here. They're fine for your goal.
You can add NaF and/or sodium orthivanadate is you matter.

i want to extract protein from heart tissue.is the extraction same to from cells?

-july-

honnestly i've never applied it to whole tissue.

-fred_33-