Any body knows the characters of plasmid pINT007 - (Jul/11/2006 )

I want to use pINT007 for disrupting Escherichia coli gene,but little is known about the vector. does anybody use this vector before?
looking forward to your answer. Great thanks !

hfrank

-hfrank-

May b this paper helps.

A rapid method for disrupting genes in the Escherichia coli genome.
Kato C, Ohmiya R, Mizuno T. Biosci Biotechnol Biochem. 1998 Sep;62(9):1826-9.

-scolix-

QUOTE (scolix @ Jul 11 2006, 07:12 AM)

May b this paper helps.

A rapid method for disrupting genes in the Escherichia coli genome.
Kato C, Ohmiya R, Mizuno T. Biosci Biotechnol Biochem. 1998 Sep;62(9):1826-9.

Thank you for your attention and good help! I've got this paper but little is known about the size of the vector and the MCS. Thank you again!

hrank

-hfrank-

if its feasible.

U could try to sequence using standard primers like T7 promoter or Sp6.

Also u could try digesting with one or more enzymes to give u an approximate idea abt the size.

-scolix-

Thank you! I'll have a try

-hfrank-