debris in immunocytochemistry... - how to get rid of them???? (Jun/29/2006 )

hi all, im new to this....im doing immunocytochemistry and am getting a lot of debris.....just cant take any picture.. ,....i dont think it is the problem with primary or secondary antibodies....maybe my blocking or antibody diluent solution..... ....how can I make things clean??? thanx a lot!

-Kathy-

QUOTE (Kathy @ Jun 29 2006, 09:27 PM)

hi all, im new to this....im doing immunocytochemistry and am getting a lot of debris.....just cant take any picture..

,....i dont think it is the problem with primary or secondary antibodies....maybe my blocking or antibody diluent solution.....

....how can I make things clean??? thanx a lot!

filter your blocking or diluent solution to get rid of debris.

-Minnie Mouse-

thanx minnie that was what i was thinking about.....any type of filter will do no?

-Kathy-

QUOTE (Kathy @ Jun 29 2006, 09:58 PM)

thanx minnie that was what i was thinking about.....any type of filter will do no?

5.0uM filter

-Minnie Mouse-