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preservation of bacteria at -20deg.c - (Jun/29/2006 )

may be this is a very simple and stupid question to you but how to preserve bacteria at -20deg.c? for all bacterial is the method same?


15% glycerol final concentration for all the species I have worked with (enterobacteria nad streptomyctes mainly) but usually -70ÂșC is advised.


We store all bacteria in -80C.