cDNA -mRNA hybrid - (Jun/28/2006 )
what the NaOH traitement to separate cDNA-mRNA hybrid after cDNA first strand synthesis.
what goal are you trying to accomplish?
I wont to polyA tail first strand cDNA with terminal transferase and using oligidT for the second strand synthesis and for PCR amplification of the cDNA pool.
najib, I have not added a polyA tail and so cannot answer that question
however, if you want to do PCR on the hybrids, I think they would probably work just fine as they are? or, use an RNase treatment
oh, and I just saw your PM. please note that I am not here all day every day; I certainly haven't been ignoring you
Can't you use an RnasH positive Reverse transcriptase? They should give you single stranded cDNA and destroy the RNA durin RT?